Product nameAnti-Geminin antibody
See all Geminin primary antibodies
DescriptionRabbit polyclonal to Geminin
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Full length protein corresponding to Human Geminin.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferConstituent: Water
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionInhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Sequence similaritiesBelongs to the geminin family.
Developmental stageAbsent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
- Information by UniProt
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
All lanes : Anti-Geminin antibody (ab175799)
Lane 1 : Control si
Lane 2 : Geminin si
Lane 3 : NIH 3T3 cell lysate at 40 µg
Predicted band size: 24 kDa
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Geminin with ab175799 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Geminin, CyclinB, DAPI, Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling Geminin with ab175799 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Middle - 10X, Right - 20X.
Immunoprecipitation analysis of U2OS cells labelling Geminin with ab175799. Protein A beads were crosslinked to the serum.
This product has been referenced in:
- Hernández-Pérez S et al. DUB3 and USP7 de-ubiquitinating enzymes control replication inhibitor Geminin: molecular characterization and associations with breast cancer. Oncogene N/A:N/A (2017). WB ; Human . Read more (PubMed: 28288134) »
- Nagano T et al. Cell-cycle dynamics of chromosomal organization at single-cell resolution. Nature 547:61-67 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28682332) »