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Full length protein corresponding to Human Geminin.
Our Abpromise guarantee covers the use of ab175799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Geminin with ab175799 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Geminin, CyclinB, DAPI, Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling Geminin with ab175799 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Middle - 10X, Right - 20X.
Immunoprecipitation analysis of U2OS cells labelling Geminin with ab175799. Protein A beads were crosslinked to the serum.
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