Overview

  • Product name
  • Description
    Rabbit polyclonal to Geminin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Geminin.

Properties

Applications

Our Abpromise guarantee covers the use of ab175799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/1000.

Target

  • Function
    Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
  • Sequence similarities
    Belongs to the geminin family.
  • Developmental stage
    Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA replication inhibitor antibody
    • Gem antibody
    • GEMI_HUMAN antibody
    • Geminin antibody
    • Geminin DNA replication inhibitor antibody
    • GMNN antibody
    • OTTHUMP00000039393 antibody
    • RP3 369A17.3 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Geminin with ab175799 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Geminin, CyclinB, DAPI, Merge.

  • Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling Geminin with ab175799 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Middle - 10X, Right - 20X.

  • Immunoprecipitation analysis of U2OS cells labelling Geminin with ab175799. Protein A beads were crosslinked to the serum.

  • All lanes : Anti-Geminin antibody (ab175799)

    Lane 1 : Control si
    Lane 2 : Geminin si
    Lane 3 : NIH 3T3 cell lysate at 40 µg

    Predicted band size: 24 kDa

References

This product has been referenced in:
  • Hernández-Pérez S  et al. DUB3 and USP7 de-ubiquitinating enzymes control replication inhibitor Geminin: molecular characterization and associations with breast cancer. Oncogene N/A:N/A (2017). WB ; Human . Read more (PubMed: 28288134) »
  • Nagano T  et al. Cell-cycle dynamics of chromosomal organization at single-cell resolution. Nature 547:61-67 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28682332) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (4-12%, PVDF)
Loading amount
20 µg
Specification
Mouse embryonic stem cells
Username

Dr. Maud de Dieuleveult

Verified customer

Submitted Jun 25 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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