Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Geminin antibody
See all Geminin primary antibodies
DescriptionRabbit polyclonal to Geminin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Geminin aa 1-209.
MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSK RKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQY MAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVS SSTDAKPCI
Database link: O75496
- SW480, 22RV-1, HL60, HepG2, MCF7 and K562 cell line extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 24 kDa.|
FunctionInhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Sequence similaritiesBelongs to the geminin family.
Developmental stageAbsent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
- Information by UniProt
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
All lanes : Anti-Geminin antibody (ab187553) at 1/500 dilution
Lane 1 : SW480 cell line extract
Lane 2 : 22RV-1 cell line extract
Lane 3 : HL60 cell line extract
Lane 4 : HepG2 cell line extract
Lane 5 : MCF7 cell line extract
Lane 6 : K562 cell line extract
Predicted band size: 24 kDa
ab187553 has not yet been referenced specifically in any publications.