Product nameAnti-Geminin antibody
See all Geminin primary antibodies
DescriptionRabbit polyclonal to Geminin
Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rhesus monkey
Recombinant full length protein corresponding to Human Geminin aa 1-209.
MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSK RKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQY MAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVS SSTDAKPCI
Database link: O75496
- WB: HCT 116 whole cell lysate. IP: HCT 116 whole cell extract. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 24 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
FunctionInhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Sequence similaritiesBelongs to the geminin family.
Developmental stageAbsent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
- Information by UniProt
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
Anti-Geminin antibody (ab229009) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS-PAGE gel.
Geminin was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell extract with 5 μg of ab229009. Western blot was performed from the immunoprecipitate using ab229009. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229009 in HCT 116 whole cell etract.
Lane 2: ab229009 IP in HCT116 whole cell extract.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Geminin (green) using ab229009 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Paraffin-embedded human breast cancer tissue stained for Geminin using ab229009 at 1/250 dilution in immunohistochemical analysis.
ab229009 has not yet been referenced specifically in any publications.