Immunocytochemistry/ Immunofluorescence abreview for Anti-Geminin antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. The antibody failed to produce the expected staining pattern under these conditions. MeOH fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. ab84423 has only been used to detect Geminin in Human tissue sections by IHC-P. Your data suggests that ab84423 may not be suitable to detect endogenous Geminin by ICC in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.

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Submitted Nov 15 2013

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