Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. The antibody failed to produce the expected staining pattern under these conditions. MeOH fixed samples were also evaluated and failed to produce the expected staining pattern.
Thank you for your valuable feedback. ab84423 has only been used to detect Geminin in Human tissue sections by IHC-P. Your data suggests that ab84423 may not be suitable to detect endogenous Geminin by ICC in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
Abcam user community
Submitted Nov 15 2013
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