Overview

  • Product name

    Anti-GEN1 antibody [EPR16863]
    See all GEN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16863] to GEN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human GEN1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q17RS7

  • Positive control

    • WB: HeLa, 293, SH-SY5Y, MDA-MB-435S and C6 whole cell lysates; rat kidney tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

Target

  • Function

    Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
  • Sequence similarities

    Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781F0986 antibody
    • Flap endonuclease GEN homolog 1 antibody
    • FLJ40869 antibody
    • GEN 1 antibody
    • Gen antibody
    • Gen homolog 1 endonuclease (Drosophila) antibody
    • Gen homolog 1 endonuclease antibody
    • GEN_HUMAN antibody
    • GEN1 antibody
    see all

Images

  • All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : C6 (rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

    Exposure time: 3 minutes

  • All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (human embryonic kidney) whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow)
    Lane 4 : MDA-MB-435S (human ductal carcinoma cell line)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

    Exposure time: 1 minute

References

ab198989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells (CCE))
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
3 µg
Specification
Mouse embryonic stem cells (CCE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up