Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16863] to GEN1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Product nameAnti-GEN1 antibody [EPR16863] - BSA and Azide free
See all GEN1 primary antibodies
DescriptionRabbit monoclonal [EPR16863] to GEN1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human GEN1 aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: Q17RS7
- WB: HeLa, 293, SH-SY5Y and MDA-MB-435S whole cell lysates.
Ab239013 is the carrier-free version of ab198989. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239013 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
FunctionEndonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. GEN subfamily.
- Information by UniProt
- DKFZp781F0986 antibody
- Flap endonuclease GEN homolog 1 antibody
- FLJ40869 antibody
All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (human embryonic kidney) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198989).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239013 has not yet been referenced specifically in any publications.