Product nameAnti-GEN1 antibody [EPR16863] - BSA and Azide free
See all GEN1 primary antibodies
DescriptionRabbit monoclonal [EPR16863] to GEN1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human GEN1 aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: Q17RS7
- WB: HeLa, 293, SH-SY5Y and MDA-MB-435S whole cell lysates.
Ab239013 is the carrier-free version of ab198989. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239013 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
FunctionEndonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. GEN subfamily.
- Information by UniProt
- DKFZp781F0986 antibody
- Flap endonuclease GEN homolog 1 antibody
- FLJ40869 antibody
All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (human embryonic kidney) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198989).
ab239013 has not yet been referenced specifically in any publications.