Overview

  • Product name

    Anti-GEN1 antibody [EPR16863] - BSA and Azide free
    See all GEN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16863] to GEN1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human GEN1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q17RS7

  • Positive control

    • WB: HeLa, 293, SH-SY5Y and MDA-MB-435S whole cell lysates.
  • General notes

    Ab239013 is the carrier-free version of ab198989. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239013 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

Target

  • Function

    Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
  • Sequence similarities

    Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781F0986 antibody
    • Flap endonuclease GEN homolog 1 antibody
    • FLJ40869 antibody
    • GEN 1 antibody
    • Gen antibody
    • Gen homolog 1 endonuclease (Drosophila) antibody
    • Gen homolog 1 endonuclease antibody
    • GEN_HUMAN antibody
    • GEN1 antibody
    see all

Images

  • All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (human embryonic kidney) whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 4 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

    Exposure time: 1 minute.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198989).

References

ab239013 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab239013.
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