Product nameGenipin, Cell-permeable uncoupling protein 2 inhibitor
DescriptionCell-permeable uncoupling protein 2 inhibitor
Biological descriptionCell-permeable uncoupling protein 2 (UCP2) inhibitor. Anti-inflammatory, anti-angiogenic and pro-apototic effects. Acts as a protein cross-linker.
Chemical name(1R,4aS,7aS)-1,4a,5,7a-Tetrahydro-1-hydroxy-7-(hydroxymethyl)cyclopenta[c]pyran-4-carboxylic acid methyl ester
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab141049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab28 staining p53 in HeLa cells treated with genipin (ab141049), by ICC/IF. Increase of p53 expression correlates with increased concentration of genipin, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab141049 (genipin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab28 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Parton LE et al. Glucose sensing by POMC neurons regulates glucose homeostasis and is impaired in obesity. Nature 449:228-32 (2007). Read more (PubMed: 17728716) »
- Zhang CY et al. Genipin inhibits UCP2-mediated proton leak and acutely reverses obesity- and high glucose-induced beta cell dysfunction in isolated pancreatic islets. Cell Metab 3:417-27 (2006). Read more (PubMed: 16753577) »
- Kim BC et al. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem Pharmacol 70:1398-407 (2005). Read more (PubMed: 16143311) »