Overview

  • Product name

  • Description

    Sheep polyclonal to Gentamycin
  • Host species

    Sheep
  • Tested applications

    Suitable for: ELISA, Competitive ELISA, Othermore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Gentamycin conjugated to BSA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.88% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab123989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1.25 µg/ml.
Competitive ELISA Use a concentration of 1.25 µg/ml. 10ng/ml Gentamycin produces 89% inhibition in a competitive ELISA employing ab123989.
Other Use a concentration of 1.25 µg/ml. ab123989 is suitable for the Development of Rapid tests and other immunoassay, antibody recognition assays.

Target

  • Relevance

    Gentamycin is an aminoglycoside antibiotic used to treat infections caused by many different types of bacteria. Gentamycin is usually administered by intravenous infusion or intramuscular injection.
  • Alternative names

    • Gentamicin antibody

References

ab123989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

The gentamycin itself has a very low molecular weight andI unfortunatelydo not know how you could perform a Western blot with this (0.5kD). You would need to conjugate the Gentamycin to a carrier protein in order to be able to detect it in Western blot. Then the expected molecular weight would be the molecular weight of the carrier protein.
http://www.drugbank.ca/drugs/DB00798

I can confirm that the antibody does recognise free Gentamycin in the assay performed in the lab. No other commercial sources of Gentamycin were tested.Additionnally, we can confirm that this antibodydoes recognise the HRP-conjugated hapten in the competitive ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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