Key features and details
- Chicken polyclonal to Gephyrin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgY
Product nameAnti-Gephyrin antibody
See all Gephyrin primary antibodies
DescriptionChicken polyclonal to Gephyrin
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide corresponding to Human Gephyrin aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive result in IHC in the following FFPE tissue: Rat normal brain. IF/ICC: MEF1 cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab136343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMicrotubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
PathwayCofactor biosynthesis; molybdopterin biosynthesis.
Involvement in diseaseDefects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
Sequence similaritiesIn the N-terminal section; belongs to the moaB/mog family.
In the C-terminal section; belongs to the moeA family.
Cellular localizationCell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
- Information by UniProt
- Domain E antibody
- Domain G antibody
- GEPH antibody
IHC-P image of Gephyrin staining on rat Caudate putamen sections using ab136343 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval uisng citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab136343 was diluted 1:100 and incubated with the section for 16 hours at 21°C. The secondary antibody used was ab6876 - Goat polyclonal to Chicken IgY H&L conjugated to Biotin (1:200)
IHC-P image of Gephyrin staining on mouse Caudate putamen sections using ab136343 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval uisng citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab136343 was diluted 1:100 and incubated with the section for 16 hours at 21°C. The secondary antibody used was ab6876 - Goat polyclonal to Chicken IgY H&L conjugated to Biotin (1:200)
ICC/IF image of ab136343 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of Gephyrin staining in Rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136343, 5µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab136343 has not yet been referenced specifically in any publications.