Anti-Gephyrin antibody (ab185993)
Key features and details
- Rabbit polyclonal to Gephyrin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Gephyrin antibody
See all Gephyrin primary antibodies -
Description
Rabbit polyclonal to Gephyrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human Gephyrin aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001019389.1.
Database link: Q9NQX3 -
Positive control
- HeLa, 293T and Jurkat cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab185993 was affinity purified using an epitope specific to Gephyrin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. -
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis. -
Involvement in disease
Defects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. -
Sequence similarities
In the N-terminal section; belongs to the moaB/mog family.
In the C-terminal section; belongs to the moeA family. -
Cellular localization
Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes. - Information by UniProt
-
Database links
- Entrez Gene: 428878 Chicken
- Entrez Gene: 535194 Cow
- Entrez Gene: 490739 Dog
- Entrez Gene: 100715524 Guinea pig
- Entrez Gene: 10243 Human
- Entrez Gene: 268566 Mouse
- Entrez Gene: 100153443 Pig
- Entrez Gene: 64845 Rat
see all -
Alternative names
- Domain E antibody
- Domain G antibody
- GEPH antibody
see all
Images
-
Detection of Gephyrin in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185993 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab185993 was used at 1µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
-
All lanes : Anti-Gephyrin antibody (ab185993) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab185993 has been referenced in 1 publication.
- Brenet A et al. Defective Excitatory/Inhibitory Synaptic Balance and Increased Neuron Apoptosis in a Zebrafish Model of Dravet Syndrome. Cells 8:N/A (2019). PubMed: 31590334