Overview

  • Product name

  • Description

    Rabbit polyclonal to Gephyrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Gephyrin (internal sequence). The exact sequence is proprietary.
    Database link: Q9NQX3

  • Positive control

    • IHC-Fr: E13.5 rat brain tissue. WB: U-87 MG, SK-N-SH, IMR32, NIH/3T3, JC and SK-N-AS whole cell extracts; mouse brain tissue extract; rat brain tissue extract. IHC-P: Mouse brain tissue. IP: A431 whole cell extract. ICC/IF: DIV10 rat E18 primary hippocampal neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab228674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 80 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
  • Pathway

    Cofactor biosynthesis; molybdopterin biosynthesis.
  • Involvement in disease

    Defects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
    Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • Sequence similarities

    In the N-terminal section; belongs to the moaB/mog family.
    In the C-terminal section; belongs to the moeA family.
  • Cellular localization

    Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Domain E antibody
    • Domain G antibody
    • GEPH antibody
    • GEPH_HUMAN antibody
    • GPH antibody
    • GPHN antibody
    • GPHRYN antibody
    • KIAA1385 antibody
    • Molybdopterin molybdenumtransferase antibody
    • MPT adenylyltransferase antibody
    • MPT Mo-transferase antibody
    see all

Images

  • All lanes : Anti-Gephyrin antibody (ab228674) at 1/3000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extracts
    Lane 3 : IMR32 (human neuroblast cell line) whole cell extracts
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 80 kDa



    7.5% SDS-PAGE

  • Frozen sectioned E13.5 rat brain tissue stained for Gephyrin with ab228674 at 1/250 dilution (green) in immunohistochemical analysis.

    Beta Tubulin 3/ TUJ1, a mature neuron marker, is detected with a beta Tubulin 3/ TUJ1 antibody (red). Nuclear counterstain: Fluoroshield with DAPI (blue).

  • Paraffin-embedded mouse brain tissue stained for Gephyrin with ab228674 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Gephyrin antibody (ab228674) at 1/5000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 80 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-Gephyrin antibody (ab228674) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 80 kDa



    7.5% SDS-PAGE

  • Gephyrin was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab228674. Western blot was performed from the immunoprecipitate using ab228674.

    Lane 2: Control IgG IP in A431 whole cell extract.

    Lane 1: ab228674 IP in A431 whole cell extract.

  • DIV10 rat E18 primary hippocampal neurons stained for Gephyrin (green) using ab228674 (1:500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody  diluted at 1:500 (red). Fluoroshield with DAPI (blue).

References

ab228674 has not yet been referenced specifically in any publications.

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