Product nameAnti-Gephyrin antibody [EPR12651(B)]
See all Gephyrin primary antibodies
DescriptionRabbit monoclonal [EPR12651(B)] to Gephyrin
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Gephyrin aa 700 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NQX3
- Human fetal brain, 293T, Jurkat, SH-SY5Y, C6, Raw264.7 and PC12 lysates. Human brain tissue. Permeabilized 293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 80 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMicrotubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
PathwayCofactor biosynthesis; molybdopterin biosynthesis.
Involvement in diseaseDefects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
Sequence similaritiesIn the N-terminal section; belongs to the moaB/mog family.
In the C-terminal section; belongs to the moeA family.
Cellular localizationCell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
- Information by UniProt
- Domain E antibody
- Domain G antibody
- GEPH antibody
All lanes : Anti-Gephyrin antibody [EPR12651(B)] (ab177154) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GPHN (Gephyrin) knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab177154 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab177154 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. Ab177154 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Gephyrin with ab177154 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometric analysis of permeabilized 293T cells labeling Gephyrin with ab177154 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
All lanes : Anti-Gephyrin antibody [EPR12651(B)] (ab177154) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lane 4 : SH-SY5Y lysate
Lane 5 : C6 lysate
Lane 6 : Raw264.7 lysate
Lane 7 : PC12 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
ab177154 has not yet been referenced specifically in any publications.