Product nameAnti-Germ cell-specific antigen antibody [TRA98]
DescriptionRat monoclonal [TRA98] to Germ cell-specific antigen
SpecificityIn PubMed: 27718356 the epitope of ab82527 has been mapped to a murine-specific 8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat that occurs 25 times in mouse GCNA1 (aa 167- 383).
Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse Germ cell-specific antigen. Cell lysates of adult mouse testes.
- Testicular germ cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 50% Glycerol
Concentration information loading...
Purification notesThis product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.
Our Abpromise guarantee covers the use of ab82527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/5000. Predicted molecular weight: 146 kDa.|
RelevanceDetected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
- GENA antibody
- Testicular germ cell-specific antigen antibody
ab82527 staining Germ cell-specific antigen in mouse postnatal day 7 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 45 minutes at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA) for 1 hour at 25°C. A biotin-conjugated goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
ab82527 staining Germ cell-specific antigen in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150165 was used as the secondary antibody, undiluted.
All lanes : Anti-Germ cell-specific antigen antibody [TRA98] (ab82527) at 0.1 µg/ml
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
Lane 3 : mouse intestine lysate
Lane 4 : mouse kidney lysate
Lane 5 : mouse liver lysate
Lane 6 : mouse lung lysate
Lane 7 : mouse spleen lysate
Lane 8 : mouse ovary lysate
Lane 9 : mouse testis lysate
Lysates/proteins at 100 µg per lane.
Predicted band size: 146 kDa
Western blot analysis of various mouse tissues, using ab82527.
In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
Immunohistochemical analysis of mouse fetal ovary tissue, staining Germ cell-specific antigen with ab82527.
Tissue was fixed with Bouin's fixative, permeabilized with 0.05% Tween 20 and 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA and 0.5% Triton X-100 in 1X PBS) for 1 hour at 25°C. An AlexaFluor®488-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.
This product has been referenced in:
- Zhou H et al. The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation. Genesis 57:e23295 (2019). Read more (PubMed: 31001916) »
- Li XY et al. Regulation of blood-testis barrier assembly in vivo by germ cells. FASEB J 32:1653-1664 (2018). Read more (PubMed: 29183964) »