Overview

  • Product name

    Anti-Germinal Center Kinase/GCK antibody
    See all Germinal Center Kinase/GCK primary antibodies
  • Description

    Rabbit polyclonal to Germinal Center Kinase/GCK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Germinal Center Kinase/GCK aa 386-469.
    Database link: Q12851

  • Positive control

    • IHC-P: Human endometrial cancer tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as Germinal Center Kinase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab238843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
  • Tissue specificity

    Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Basolateral cell membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B lymphocyte serine/threonine protein kinase antibody
    • B lymphocyte serine/threonine-protein kinase antibody
    • BL44 antibody
    • GC kinase antibody
    • GCK antibody
    • Germinal center kinase antibody
    • germinal centre kinase (GC kinase) antibody
    • M4K2_HUMAN antibody
    • Map4k2 antibody
    • MAPK/ERK kinase kinase kinase 2 antibody
    • MEK kinase kinase 2 antibody
    • MEKKK 2 antibody
    • Mitogen activated protein kinase kinase kinase kinase 2 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 2 antibody
    • Rab8 interacting protein antibody
    • Rab8 interacting protein, formerly antibody
    • Rab8-interacting protein antibody
    • RAB8IP antibody
    • RAB8IP, formerly antibody
    see all

Images

  • Paraffin-embedded human endometrial cancer tissue stained for Germinal Center Kinase/GCK using ab238843 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Germinal Center Kinase/GCK (Green) using ab238843 at 1/166 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain is DAPI (Blue).

     

References

ab238843 has not yet been referenced specifically in any publications.

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