Overview

  • Product name

    Anti-GFAP antibody [5C10]
    See all GFAP primary antibodies
  • Description

    Mouse monoclonal [5C10] to GFAP
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Horse, Chicken, Cow, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Pig GFAP. Purified form Pig spinal cord.

  • Positive control

    • Rat spinal cord protein extract; Mixed neuron-glial culture; Adult horse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab190288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/5000. Predicted molecular weight: 49 kDa.
IHC-Fr 1/1000.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity

    Expressed in cells lacking fibronectin.
  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylated by PKN1.
  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links

  • Alternative names

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed adult horse brain labeling GFAP with ab190288 at 1/1000 dilution.

  • Immunofluorescent analysis of mixed neuron-glial cultures labeling GFAP with ab190288 at 1/1000 dilution. Neurofilaments are stained in green; the blue channel shows the localization of DNA. 

  • Anti-GFAP antibody [5C10] (ab190288) at 1/5000 dilution + Rat spinal cord protein extract

    Predicted band size: 49 kDa

References

This product has been referenced in:

  • Shi A  et al. The Phenolic Components of Gastrodia elata improve Prognosis in Rats after Cerebral Ischemia/Reperfusion by Enhancing the Endogenous Antioxidant Mechanisms. Oxid Med Cell Longev 2018:7642158 (2018). Read more (PubMed: 29765502) »
  • Zhang J  et al. LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/ß-catenin pathway and predicts poor survival of glioma patients. J Exp Clin Cancer Res 37:225 (2018). Read more (PubMed: 30208924) »
See all 2 Publications for this product

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