Key features and details
- Chicken polyclonal to GFAP
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgY
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-GFAP antibody
See all GFAP primary antibodies
DescriptionChicken polyclonal to GFAP
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Rat
Recombinant full length protein followed by boosts of native GFAP protein purified from bovine spinal cords
General notesDo not freeze this antibody unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA
Concentration information loading...
Purification notesAfter repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificityExpressed in cells lacking fibronectin.
Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylated by PKN1.
Cellular localizationCytoplasm. Associated with intermediate filaments.
- Information by UniProt
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Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.
ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.
ab134436 has been referenced in 9 publications.
- Eder N et al. YAP1/TAZ drives ependymoma-like tumour formation in mice. Nat Commun 11:2380 (2020). PubMed: 32404936
- Woodruff G et al. Screening for modulators of neural network activity in 3D human iPSC-derived cortical spheroids. PLoS One 15:e0240991 (2020). PubMed: 33091047
- Chen PA et al. Absent in melanoma 2 regulates tumor cell proliferation in glioblastoma multiforme. J Neurooncol 144:265-273 (2019). PubMed: 31280432
- Han F et al. Therapeutic potential of a TrkB agonistic antibody for ischemic brain injury. Neurobiol Dis 127:570-581 (2019). PubMed: 30981830
- Alves MJ et al. CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus. Am J Pathol 189:426-439 (2019). PubMed: 30579783
- Gilmour A et al. An Improved in vitro Model of Cortical Tissue. Front Neurosci 13:1349 (2019). PubMed: 31920510
- Nie SD et al. High glucose forces a positive feedback loop connecting ErbB4 expression and mTOR/S6K pathway to aggravate the formation of tau hyperphosphorylation in differentiated SH-SY5Y cells. Neurobiol Aging 67:171-180 (2018). PubMed: 29674181
- Chen Z et al. The autophagic degradation of Cav-1 contributes to PA-induced apoptosis and inflammation of astrocytes. Cell Death Dis 9:771 (2018). PubMed: 29991726
- Freeman L et al. NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes. J Exp Med 214:1351-1370 (2017). ICC/IF . PubMed: 28404595
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