Anti-GFAP antibody (ab16997)
- Datasheet
- References (24)
- Protocols
Overview
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Product nameAnti-GFAP antibody
See all GFAP primary antibodies -
DescriptionRabbit polyclonal to GFAP
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, ICC, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
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Immunogen
Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.
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Positive control
- ICC/IF: Mouse cortical primary astrocytes. IHC-P: Human brain tissue.
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General notes
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityIgG fraction
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Primary antibody notesIn the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab16997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24223835 | |
| ICC | 1/100. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20460993 | |
| IHC-P | 1/50 - 1/250. |
Target
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FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Tissue specificityExpressed in cells lacking fibronectin.
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Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
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Sequence similaritiesBelongs to the intermediate filament family.
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Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localizationCytoplasm. Associated with intermediate filaments.
- Information by UniProt
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Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
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Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
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Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.
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Immunocytochemistry - Anti-GFAP antibody (ab16997)Photo courtesy of QBMCellScienceMouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)Formalin-fixed, paraffin-embedded section of brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
Datasheets and documents
References
This product has been referenced in:
- Sanderson TH et al. Inhibitory modulation of cytochrome c oxidase activity with specific near-infrared light wavelengths attenuates brain ischemia/reperfusion injury. Sci Rep 8:3481 (2018). Read more (PubMed: 29472564) »
- Kuwahara H et al. Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo. Sci Rep 8:4377 (2018). IHC . Read more (PubMed: 29531265) »
