Anti-GFAP antibody (ab16997)
Key features and details
- Rabbit polyclonal to GFAP
- Suitable for: IHC-Fr, ICC, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Sheep, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
- Isotype: IgG
Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Rabbit polyclonal to GFAP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey -
Immunogen
Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.
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Positive control
- ICC/IF: Mouse cortical primary astrocytes. IHC-P: Human brain tissue.
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General notes
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Primary antibody notes
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab16997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24223835 | |
| ICC | 1/100. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20460993 | |
| IHC-P | 1/50 - 1/250. |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.
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Immunocytochemistry - Anti-GFAP antibody (ab16997)Photo courtesy of QBMCellScienceMouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)Formalin-fixed, paraffin-embedded section of human brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
Datasheets and documents
References
ab16997 has been referenced in 33 publications.
- Chunchai T et al. N-acetyl cysteine, inulin and the two as a combined therapy ameliorate cognitive decline in testosterone-deprived rats. Aging (Albany NY) 11:3445-3462 (2019). PubMed: 31160542
- Adlimoghaddam A et al. Regional hypometabolism in the 3xTg mouse model of Alzheimer's disease. Neurobiol Dis 127:264-277 (2019). PubMed: 30878533
- Zhao L et al. Administration of rCTRP9 Attenuates Neuronal Apoptosis Through AdipoR1/PI3K/Akt Signaling Pathway after ICH in Mice. Cell Transplant 28:756-766 (2019). PubMed: 30642187
- Ellsworth JL et al. Clade F AAVHSCs cross the blood brain barrier and transduce the central nervous system in addition to peripheral tissues following intravenous administration in nonhuman primates. PLoS One 14:e0225582 (2019). PubMed: 31770409
- Yang L et al. Efficient Synthesis of Light-Triggered Circular Antisense Oligonucleotides Targeting Cellular Protein Expression. Chembiochem 19:1250-1254 (2018). PubMed: 29479781
