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    gfap-antibody-ab16997.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Anti-GFAP antibody (ab16997)

  • Datasheet
Reviews (1)Q&A (2)References (48)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)
  • Immunocytochemistry - Anti-GFAP antibody (ab16997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)

Key features and details

  • Rabbit polyclonal to GFAP
  • Suitable for: ICC, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GFAP antibody
    See all GFAP primary antibodies
  • Description

    Rabbit polyclonal to GFAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.

  • General notes


    In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Primary antibody notes

    In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
  • Related Products

    • Human GFAP Matched Antibody Pair Kit (ab222279)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/100.
IHC-P (1)
1/50 - 1/250.
Notes
ICC
1/100.
IHC-P
1/50 - 1/250.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity

    Expressed in cells lacking fibronectin.
  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylated by PKN1.
  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2670 Human
    • Entrez Gene: 14580 Mouse
    • Omim: 137780 Human
    • SwissProt: P14136 Human
    • SwissProt: P03995 Mouse
    • Unigene: 514227 Human
    • Unigene: 1239 Mouse
    • Alternative names

      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.

    • Immunocytochemistry - Anti-GFAP antibody (ab16997)
      Immunocytochemistry - Anti-GFAP antibody (ab16997)Photo courtesy of QBMCellScience

      Mouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)

      Formalin-fixed, paraffin-embedded section of human brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (48)

    Publishing research using ab16997? Please let us know so that we can cite the reference in this datasheet.

    ab16997 has been referenced in 48 publications.

    • Adlimoghaddam A  et al. Nilotinib Improves Bioenergetic Profiling in Brain Astroglia in the 3xTg Mouse Model of Alzheimer's Disease. Aging Dis 12:441-465 (2021). PubMed: 33815876
    • Deng S  et al. Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice. J Neuroinflammation 18:62 (2021). PubMed: 33648537
    • Han GH  et al. Transplantation of tauroursodeoxycholic acid-inducing M2-phenotype macrophages promotes an anti-neuroinflammatory effect and functional recovery after spinal cord injury in rats. Cell Prolif 54:e13050 (2021). PubMed: 33960559
    • Sick F  et al. Shuni virus-induced meningoencephalitis after experimental infection of cattle. Transbound Emerg Dis 68:1531-1540 (2021). PubMed: 32910551
    • Zhang L  et al. BAD-mediated neuronal apoptosis and neuroinflammation contribute to Alzheimer's disease pathology. iScience 24:102942 (2021). PubMed: 34430820
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody - Astrocyte Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 22 2007

    Question

    I'm wanting to immunostain protoplasmic astrocytes in human brain paraffin sections.

    Can you tell me which of your antibodies would be best for this? Is there any published work in this area.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 13 2012

    Answer

    After completing a short literature search, GFAP is commonly used as an protoplasmic astrocyte marker. Below are two references:

    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038243
    http://www.jneurosci.org/content/22/1/183.full.pdf

    We stock 30 antibodies that are tested in IHC on human cells.

    Read More

    Abcam Scientific Support

    Answered on Sep 13 2012

    Question

    Customer kindly asked if the antibody ab16997 will not detect oligodendrocytes or micgliocytes.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2011

    Answer

    I can confirm that ab16997 will only detect GFAP in astorcytes not in oligodendrocytes and micogliocytes (these cells do not express this protein).

    Read More

    Abcam Scientific Support

    Answered on Nov 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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