Anti-GFAP antibody (ab16997)
Key features and details
- Rabbit polyclonal to GFAP
- Suitable for: ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Rabbit polyclonal to GFAP -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.
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General notes
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Primary antibody notes
In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100.
|
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| IHC-P | (1) |
1/50 - 1/250.
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| Notes |
|---|
|
ICC
1/100. |
|
IHC-P
1/50 - 1/250. |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- Unigene: 514227 Human
- Unigene: 1239 Mouse
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Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.
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Immunocytochemistry - Anti-GFAP antibody (ab16997)Photo courtesy of QBMCellScienceMouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab16997)Formalin-fixed, paraffin-embedded section of human brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
Protocols
Datasheets and documents
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Datasheet download
References (36)
ab16997 has been referenced in 36 publications.
- Gao J et al. Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice. Aging (Albany NY) 12:17738-17753 (2020). PubMed: 32920547
- Dixit S et al. PEDF deficiency increases the susceptibility of rd10 mice to retinal degeneration. Exp Eye Res 198:108121 (2020). PubMed: 32721425
- Butt SL et al. Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia. BMC Vet Res 15:317 (2019). PubMed: 31484573
- Chunchai T et al. N-acetyl cysteine, inulin and the two as a combined therapy ameliorate cognitive decline in testosterone-deprived rats. Aging (Albany NY) 11:3445-3462 (2019). PubMed: 31160542
- Adlimoghaddam A et al. Regional hypometabolism in the 3xTg mouse model of Alzheimer's disease. Neurobiol Dis 127:264-277 (2019). PubMed: 30878533
Customer reviews and Q&As
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