For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    gfap-antibody-ab53554.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
Share by email

Anti-GFAP antibody (ab53554)

  • Datasheet
  • SDS
Reviews (24) Submit a question References (156)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GFAP antibody (ab53554)
  • Western blot - Anti-GFAP antibody (ab53554)
  • Western blot - Anti-GFAP antibody (ab53554)

Key features and details

  • Goat polyclonal to GFAP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human GFAP protein (ab114149)
Primary
Product image
Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
Primary
Product image
Anti-MAP2 antibody [EPR22036-127] (ab221693)

View more associated products

Overview

  • Product name

    Anti-GFAP antibody
    See all GFAP primary antibodies
  • Description

    Goat polyclonal to GFAP
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GFAP aa 400 to the C-terminus (C terminal) (Cysteine residue).
    Database link: P14136-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human cerebellum and cerebral cortex lysates. Mouse and rat brain lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
    • Recombinant Human GFAP protein (ab151370)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Human GFAP Matched Antibody Pair Kit (ab222279)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
Human
All applications
Dog
Application Abreviews Notes
WB (3)
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.

Notes
WB
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity

    Expressed in cells lacking fibronectin.
  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylated by PKN1.
  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 480495 Dog
    • Entrez Gene: 2670 Human
    • Entrez Gene: 14580 Mouse
    • Entrez Gene: 24387 Rat
    • Omim: 137780 Human
    • SwissProt: P14136 Human
    • SwissProt: P03995 Mouse
    • SwissProt: P47819 Rat
    • Unigene: 514227 Human
    • Unigene: 1239 Mouse
    • Unigene: 91512 Rat
    see all
  • Alternative names

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • Western blot - Anti-GFAP antibody (ab53554)
    Western blot - Anti-GFAP antibody (ab53554)
    All lanes : Anti-GFAP antibody (ab53554) at 0.001 µg/ml

    Lane 1 : Human cerebellum tissue lysate (in RIPA buffer)
    Lane 2 : Human cerebral cortex tissue lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa



    Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

  • Western blot - Anti-GFAP antibody (ab53554)
    Western blot - Anti-GFAP antibody (ab53554)
    Lane 1 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
    Lane 2 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml

    Lane 1 : Mouse brain tissue lysate (in RIPA buffer)
    Lane 2 : Rat brain tissue lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa



    Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

  • Western blot - Anti-GFAP antibody (ab53554)
    Western blot - Anti-GFAP antibody (ab53554)This image was courtesy of an annonymous Abreview
    All lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution

    All lanes : Human Tissue lysate - other (Frontal Cortex)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Donkey anti-goat IRDye­ 800CW conjugated Li-Cor IRdye

    Predicted band size: 50 kDa
    Observed band size: 40-50 kDa
    why is the actual band size different from the predicted?

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (156)

    Publishing research using ab53554? Please let us know so that we can cite the reference in this datasheet.

    ab53554 has been referenced in 156 publications.

    • Kjell J  et al. Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell 26:277-293.e8 (2020). PubMed: 32032526
    • Ayanlaja AA  et al. Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Cell Commun Signal 18:24 (2020). PubMed: 32050972
    • Patrizz A  et al. EMMPRIN/CD147 plays a detrimental role in clinical and experimental ischemic stroke. Aging (Albany NY) 12:5121-5139 (2020). PubMed: 32191628
    • Cruz SL  et al. Minocycline prevents neuronal hyperexcitability and neuroinflammation in medial prefrontal cortex, as well as memory impairment caused by repeated toluene inhalation in adolescent rats. Toxicol Appl Pharmacol 395:114980 (2020). PubMed: 32234516
    • Fragola G  et al. Deletion of Topoisomerase 1 in excitatory neurons causes genomic instability and early onset neurodegeneration. Nat Commun 11:1962 (2020). PubMed: 32327659
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 24 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (astrocytes)
    Permeabilization
    No
    Specification
    astrocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 17 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (spinal cord cross section)
    Permeabilization
    Yes - 0,01% Triton-X 100
    Specification
    spinal cord cross section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Mr. Tamas Bellak

    Verified customer

    Submitted Nov 16 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Monkey Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton 1%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted May 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (Brain tissue)
    Permeabilization
    No
    Specification
    Brain tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Chiara La Rosa

    Verified customer

    Submitted Apr 30 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
    Permeabilization
    Yes - 1% Triton-X
    Specification
    brain
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Jun 13 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Permeabilization
    No
    Specification
    brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 28°C
    Fixative
    Formaldehyde
    Read More

    Miss. Regina Vontell

    Verified customer

    Submitted May 01 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFAP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Zamboni
    Read More

    Mr. Duc Dung Le

    Verified customer

    Submitted Apr 20 2015

    Western blot abreview for Anti-GFAP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Tissue lysate - other (Frontal Cortex)
    Specification
    Frontal Cortex
    Blocking step
    Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 21 2015

    Western blot abreview for Anti-GFAP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
    Specification
    total larvae, 4 days post fertilization
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Dr. Evgenia Salta

    Verified customer

    Submitted Oct 27 2014

    1-10 of 24 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • 3
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.