Anti-GFAP antibody (ab53554)
Key features and details
- Goat polyclonal to GFAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Goat polyclonal to GFAP -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human GFAP aa 400 to the C-terminus (C terminal) (Cysteine residue).
Database link: P14136-1 -
Positive control
- WB: Human cerebellum and cerebral cortex lysates. Mouse and rat brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Rat
Human
|
| All applications |
Dog
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
| Notes |
|---|
|
WB
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 480495 Dog
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Western blot - Anti-GFAP antibody (ab53554)All lanes : Anti-GFAP antibody (ab53554) at 0.001 µg/ml
Lane 1 : Human cerebellum tissue lysate (in RIPA buffer)
Lane 2 : Human cerebral cortex tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554)Lane 1 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 1 : Mouse brain tissue lysate (in RIPA buffer)
Lane 2 : Rat brain tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554)This image was courtesy of an annonymous AbreviewAll lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?
Protocols
References (156)
ab53554 has been referenced in 156 publications.
- Kjell J et al. Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell 26:277-293.e8 (2020). PubMed: 32032526
- Ayanlaja AA et al. Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Cell Commun Signal 18:24 (2020). PubMed: 32050972
- Patrizz A et al. EMMPRIN/CD147 plays a detrimental role in clinical and experimental ischemic stroke. Aging (Albany NY) 12:5121-5139 (2020). PubMed: 32191628
- Cruz SL et al. Minocycline prevents neuronal hyperexcitability and neuroinflammation in medial prefrontal cortex, as well as memory impairment caused by repeated toluene inhalation in adolescent rats. Toxicol Appl Pharmacol 395:114980 (2020). PubMed: 32234516
- Fragola G et al. Deletion of Topoisomerase 1 in excitatory neurons causes genomic instability and early onset neurodegeneration. Nat Commun 11:1962 (2020). PubMed: 32327659
