Anti-GFAP antibody (ab53554)
- Datasheet
- References (118)
- Protocols
Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Goat polyclonal to GFAP -
Host species
Goat -
Tested applications
Suitable for: IHC-FrFl, WB, IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human GFAP aa 417-430 (C terminal) (Cysteine residue).
Sequence:DGEVIKESKQEHKD
Database link: P14136-1 -
Positive control
- WB: Mouse, rat and human brain tissue lysate. IHC-P: Human brain tissue. IHC-fr: Mouse brain tissue. ICC/IF: Mongolian gerbil brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab53554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl | 1/500 - 1/2000. | |
| WB | Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21943601 |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 480495 Dog
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Entrez Gene: 30646 Zebrafish
- Omim: 137780 Human
- SwissProt: Q28115 Cow
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Western blot - Anti-GFAP antibody (ab53554)Lane 1 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
Lane 1 : Mouse brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: ~50 kDa why is the actual band size different from the predicted?Primary antibody incubation: 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab53554)This image is courtesy from an abreview submitted by Miss. Regina VontellFormalin-fixed, paraffin-embedded human brain tissue labeling GFAP using ab53554 at 1 µg/ml (green) followed by a Alexa Fluor® 488 donkey anti-goat secondary antibody at 1/500 dilution.
Antigen retreival: Heat mediated - Buffer/Enzyme Used: Citric Acid
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Western blot - Anti-GFAP antibody (ab53554)This image was courtesy of an annonymous AbreviewAll lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab53554)This is image is courtesy of an Abreview submitted by Chiara La RosaICC/IF analysis of paraformaldehyde-fixed Sheep Cell (Brain tissue) labeling GFAP (red) using ab53554 at 1/1000 dilution followed by a Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) at 1/800 dilution.
Blocking step: BSA as blocking agent for 1 hour(s) and 30 minute(s). Concentration: 2%. Temperature: RT°C
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Immunohistochemistry (Frozen sections) - Anti-GFAP antibody (ab53554)This image is courtesy of an Abreview submitted by Judith Kranzab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus. -
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab53554)Image courtesy of an anonymous Abreview.ab53554 staining GFAP in Mongolian gerbil brain tissue sections by Immunohistochemistry - Free Floating. Samples were incubated with ab53554 at a 1/500 dilution in 1% BSA, 0.3% Triton X-100, 0.1% Saponin for 16 hours at 4°C. ab6566 Donkey polyclonal Secondary Antibody to anti-goat Cy5 ® (IgG - H&L)), pre-adsorbed was used as the secondary antibody at a 1/100 dilution (green). Nuclei are counterstained with DAPI.
Protocols
References
This product has been referenced in:
- Jassim AH & Inman DM Evidence of Hypoxic Glial Cells in a Model of Ocular Hypertension. Invest Ophthalmol Vis Sci 60:1-15 (2019). Read more (PubMed: 30601926) »
- Cheng XE et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem 63:N/A (2019). Read more (PubMed: 30678436) »
