Product nameAnti-GFAP antibody
See all GFAP primary antibodies
DescriptionGoat polyclonal to GFAP
Tested applicationsSuitable for: IHC-FrFl, WB, IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Dog
- WB: Mouse, rat and human brain tissue lysate. IHC-P: Human brain tissue. IHC-fr: Mouse brain tissue. ICC/IF: Mongolian gerbil brain.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab53554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||1/500 - 1/2000.|
|WB||Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21943601|
FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificityExpressed in cells lacking fibronectin.
Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylated by PKN1.
Cellular localizationCytoplasm. Associated with intermediate filaments.
- Information by UniProt
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Lane 1 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
Lane 1 : Mouse brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: ~50 kDa why is the actual band size different from the predicted?
Primary antibody incubation: 1 hour at room temperature.
Formalin-fixed, paraffin-embedded human brain tissue labeling GFAP using ab53554 at 1 µg/ml (green) followed by a Alexa Fluor® 488 donkey anti-goat secondary antibody at 1/500 dilution.
Antigen retreival: Heat mediated - Buffer/Enzyme Used: Citric Acid
All lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?
ICC/IF analysis of paraformaldehyde-fixed Sheep Cell (Brain tissue) labeling GFAP (red) using ab53554 at 1/1000 dilution followed by a Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) at 1/800 dilution.
Blocking step: BSA as blocking agent for 1 hour(s) and 30 minute(s). Concentration: 2%. Temperature: RT°C
ab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus.
ab53554 staining GFAP in Mongolian gerbil brain tissue sections by Immunohistochemistry - Free Floating. Samples were incubated with ab53554 at a 1/500 dilution in 1% BSA, 0.3% Triton X-100, 0.1% Saponin for 16 hours at 4°C. ab6566 Donkey polyclonal Secondary Antibody to anti-goat Cy5 ® (IgG - H&L)), pre-adsorbed was used as the secondary antibody at a 1/100 dilution (green). Nuclei are counterstained with DAPI.
This product has been referenced in:
- Jassim AH & Inman DM Evidence of Hypoxic Glial Cells in a Model of Ocular Hypertension. Invest Ophthalmol Vis Sci 60:1-15 (2019). Read more (PubMed: 30601926) »
- Cheng XE et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem 63:N/A (2019). Read more (PubMed: 30678436) »