Anti-GFAP antibody (ab53554)
- Datasheet
- References (88)
- Protocols
Overview
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Product nameAnti-GFAP antibody
See all GFAP primary antibodies -
DescriptionGoat polyclonal to GFAP
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Host speciesGoat
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Tested applicationsSuitable for: IHC-FrFl, WB, Sandwich ELISA, IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human GFAP aa 417-430 (C terminal) (Cysteine residue).
Sequence:DGEVIKESKQEHKD
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Positive control
- WB: Mouse, rat and human brain tissue lysate. IHC-P: Human cerebellum tissue; Human cortex tissue. IHC-fr: Mouse brain tissue. ICC/IF: Mongolian gerbil brain.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab53554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl | 1/500 - 1/2000. | |
| WB | Use a concentration of 0.001 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa). | |
| Sandwich ELISA | Use a concentration of 5 - 10 µg/ml. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21943601 |
Target
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FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Tissue specificityExpressed in cells lacking fibronectin.
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Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
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Sequence similaritiesBelongs to the intermediate filament family.
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Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localizationCytoplasm. Associated with intermediate filaments.
- Information by UniProt
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Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 480495 Dog
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Entrez Gene: 30646 Zebrafish
- Omim: 137780 Human
- SwissProt: Q28115 Cow
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab53554)Immunohistochemical analysis of paraffin embedded Human cortex staining GFAP using ab53554 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP staining. -
Western blot - Anti-GFAP antibody (ab53554)All lanes : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa -
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody (ab53554)This image is courtesy of an Abreview submitted by Judith Kranzab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus. -
Western blot - Anti-GFAP antibody (ab53554)Anti-GFAP antibody (ab53554) at 0.1 µg/ml + Mouse Brain Lysate in RIPA Buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Primary incubation was 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab53554)Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling GFAP with ab53554 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab53554)Image courtesy of an anonymous Abreview.ab53554 staining GFAP in Mongolian gerbil brain tissue sections by Immunohistochemistry - Free Floating.Samples were incubated with ab53554 at a 1/500 dilution in 1% BSA, 0.3% Triton X-100, 0.1% Saponin for 16 hours at 4°C. ab6566 Donkey polyclonal Secondary Antibody to anti-goat Cy5 ® (IgG - H&L)), pre-adsorbed was used as the secondary antibody at a 1/100 dilution (green). Nuclei are counterstained with DAPI.
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Sandwich ELISA - Anti-GFAP antibody (ab53554)ab53554 (5ug/ml) as the reporter with a capture rabbit antibody (5ug/ml).
Protocols
References
This product has been referenced in:
- Jassim AH & Inman DM Evidence of Hypoxic Glial Cells in a Model of Ocular Hypertension. Invest Ophthalmol Vis Sci 60:1-15 (2019). Read more (PubMed: 30601926) »
- Cheng XE et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem 63:N/A (2019). Read more (PubMed: 30678436) »
