Overview

  • Product name

    Anti-GFAP antibody - Affinity Purified
    See all GFAP primary antibodies
  • Description

    Rabbit polyclonal to GFAP - Affinity Purified
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Fr, ICC/IF, IHC-FoFr, IHC-P, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to GFAP.

  • General notes

    In some cases, the antibody may appear red in color. This is due to small amounts of hemolysis, and does not affect antibody performance.

Properties

Applications

Our Abpromise guarantee covers the use of ab211271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000 - 1/5000.
WB 1/5000. Detects a band of approximately 55,48 kDa.
IHC-Fr 1/1000 - 1/5000.
ICC/IF 1/1000 - 1/5000.
IHC-FoFr 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FrFl 1/1000 - 1/5000.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity

    Expressed in cells lacking fibronectin.
  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylated by PKN1.
  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links

  • Alternative names

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • Immunohistochemistry (Free Floating) analysis of rat cerebellum staining GFAP with ab211271 (1/5000) in green and MeCP2 with a mouse monoclonal antibody (1/500) in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45μM, and free-floating sections were stained with above antibodies. 

  • Lane 1 : Protein ladder
    Lanes 2-5 : Anti-GFAP antibody - Affinity Purified (ab211271) at 1/5000 dilution

    Lane 2 : Rat brain
    Lane 3 : Rat spinal cord
    Lane 4 : Mouse brain
    Lane 5 : Mouse spinal cord


    Strong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.

References

ab211271 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211271.
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