Key features and details
- Rabbit polyclonal to GFAP - Affinity Purified
- Suitable for: ICC, WB, IHC-Fr, ICC/IF, IHC-FoFr, IHC-P, IHC-FrFl
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GFAP antibody - Affinity Purified
See all GFAP primary antibodies
DescriptionRabbit polyclonal to GFAP - Affinity Purified
Tested applicationsSuitable for: ICC, WB, IHC-Fr, ICC/IF, IHC-FoFr, IHC-P, IHC-FrFlmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to GFAP.
General notesIn some cases, the antibody may appear red in color. This is due to small amounts of hemolysis, and does not affect antibody performance.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Purification notesThe antibody was made against full length recombinant GFAP based on the human sequence expressed in and purified from E. coli.
Our Abpromise guarantee covers the use of ab211271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/1000 - 1/5000.|
|WB||1/5000. Detects a band of approximately 55,48 kDa.|
|IHC-Fr||1/1000 - 1/5000.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-FoFr||1/1000 - 1/5000.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-FrFl||1/1000 - 1/5000.|
FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificityExpressed in cells lacking fibronectin.
Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylated by PKN1.
Cellular localizationCytoplasm. Associated with intermediate filaments.
- Information by UniProt
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Immunohistochemistry (Free Floating) analysis of rat cerebellum staining GFAP with ab211271 (1/5000) in green and MeCP2 with a mouse monoclonal antibody (1/500) in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45μM, and free-floating sections were stained with above antibodies.
Lane 1 : Protein ladder
Lanes 2-5 : Anti-GFAP antibody - Affinity Purified (ab211271) at 1/5000 dilution
Lane 2 : Rat brain
Lane 3 : Rat spinal cord
Lane 4 : Mouse brain
Lane 5 : Mouse spinal cord
Strong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
ab211271 has been referenced in 1 publication.
- Nunes NS et al. Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathway. EBioMedicine 45:495-510 (2019). PubMed: 31253515