Recombinant
RabMAb

Anti-GFAP antibody [EPR1034Y] (Alexa Fluor® 647) (ab194325)

Overview

  • Product name
    Anti-GFAP antibody [EPR1034Y] (Alexa Fluor® 647)
    See all GFAP primary antibodies
  • Description
    Rabbit monoclonal [EPR1034Y] to GFAP (Alexa Fluor® 647)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human GFAP aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • IHC-Fr: Rat brain (Hypothalamus). Flow Cyt: SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

Properties

Applications

Our Abpromise guarantee covers the use of ab194325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.

Target

  • Function
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity
    Expressed in cells lacking fibronectin.
  • Involvement in disease
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localization
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • IHC-Fr image of GFAP staining in a section of frozen rat brain (hypothalamus). The section was air dried on the bench for 30 minutes and blocked for 2 hours in 1XPBS/0.1% Tween 20/1% BSA/0.3M Glycine buffer. The sample was then incubated overnight at +4°C with ab194325 at 1/50 dilution (shown in red). DAPI-containing mounting media was added to the sample (shown in blue). The inset negative control image is taken from an identical assay without primary antibody.

References

ab194325 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (mouse liver single cell suspension)
Permeabilization
Yes - saponin 0.02%
Gating Strategy
Mouse liver Live cells were gated on FS vs SS plot. Then we were interested in analyzing GFAP positive cells among live cells.
Specification
mouse liver single cell suspension
Preparation
Cell harvesting/tissue preparation method: Murine Liver single cell suspension was obtained by means of a cocktail of 0.05% w/v of Collagenase plus 0.005 ug/mL of DNase (30' at 37¯C) and subsequent 100 um cell strainer filtration.
Sample buffer: PBS for single cell suspension preparation Hanks buffer for Flow Cytometry sample preparation
Fixation
Formaldehyde
Username

Marika Crescenzi

Verified customer

Submitted Feb 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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