Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [GF5] - Astrocyte Marker

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line U87MG)
Specification
human glioblastoma cell line U87MG
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X 100 in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: 115-545-003 Alexa Fluor® 488 Goat Anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
Nucleii are stained with DAPI

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Submitted Feb 06 2012

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