Western blot abreview for Anti-GFAP antibody [GF5] - Astrocyte Marker

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Astrocytes)
Loading amount
30 µg
Specification
Astrocytes
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2500
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouseIgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 28, 36 kDa

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Submitted Feb 08 2013

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