Flow Cytometry abreview for Anti-GFAP antibody [GF5] - Astrocyte Marker

Poor
Abreviews
Application
Flow Cytometry
Fixation
Frozen
Permeabilization
Yes - 0.1% Triton-X
Sample
Human Cell (Brain)
Specification
Brain
Gating Strategy
forward scatter/side scatter (all cells)
Preparation
Cell harvesting/tissue preparation method: Homogenized frozen tissue
Sample buffer: Tris/EDTA/sucrose/Triton

Other product details

Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS with BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen goat anti-mouse 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction. We would suggest to include a reference sample such as an isotype control to be able to interpret the data better.

Dr. Mollie Woodworth

Verified customer

Submitted Sep 03 2013

Sign up