• Product name
    Anti-GFAP antibody [ZRF-1]
    See all GFAP primary antibodies
  • Description
    Mouse monoclonal [ZRF-1] to GFAP
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Does not react with: Mouse, Rat
  • Immunogen

    Zebrafish Organism.

  • Positive control
    • Human brain and Zebrafish brain homogenates.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab154474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
IP Use at an assay dependent concentration.


  • Function
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity
    Expressed in cells lacking fibronectin.
  • Involvement in disease
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    Phosphorylated by PKN1.
  • Cellular localization
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all


  • All lanes : Anti-GFAP antibody [ZRF-1] (ab154474) at 0.5 µg/ml

    Lane 1 : Human Brain Homogenate lysate
    Lane 2 : Zebrafish Brain Homogenate lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size: 51 kDa

  • Immunoprecipitation Western Blot for ab154474.
    All lanes: ab154474 at 0.5 µg/ml.
    Lane 1: Zebrafish Brain Homogenate lysate 10 µg
    Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
    Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.


This product has been referenced in:
  • Yang Q  et al. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol 3:484-499 (2017). IF ; Zebrafish . Read more (PubMed: 28462385) »
See 1 Publication for this product

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