Product nameAnti-GFI1 antibody
DescriptionRabbit polyclonal to GFI1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Dog, Xenopus laevis
- THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab21061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).Can be blocked with GFI1 peptide (ab222418).|
FunctionTranscription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
Involvement in diseaseDefects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
DomainThe Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
- Information by UniProt
- GFI 1 antibody
- GFI1 antibody
- GFI1_HUMAN antibody
All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml
Lane 1 :
THP1 whole cell lysate (ab7913)
Lane 2 :
THP1 whole cell lysate (ab7913) with Human GFI1 peptide (ab22461) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible cleavage fragment), 36 kDa (possible cross reactivity)
ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.
This product has been referenced in:
- Adamik J et al. XRK3F2 Inhibition of p62-ZZ Domain Signaling Rescues Myeloma-Induced GFI1-Driven Epigenetic Repression of the Runx2 Gene in Pre-osteoblasts to Overcome Differentiation Suppression. Front Endocrinol (Lausanne) 9:344 (2018). Read more (PubMed: 30008697) »
- Collombet S et al. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc Natl Acad Sci U S A 114:5792-5799 (2017). WB . Read more (PubMed: 28584084) »