Anti-GFI1 antibody (ab21061)
Key features and details
- Rabbit polyclonal to GFI1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GFI1 antibody -
Description
Rabbit polyclonal to GFI1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis -
Immunogen
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Positive control
- THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription. -
Involvement in disease
Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Domain
The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2672 Human
- Entrez Gene: 14581 Mouse
- Entrez Gene: 24388 Rat
- Omim: 600871 Human
- SwissProt: Q99684 Human
- SwissProt: P70338 Mouse
- SwissProt: Q07120 Rat
- Unigene: 73172 Human
see all -
Alternative names
- GFI 1 antibody
- GFI1 antibody
- GFI1_HUMAN antibody
see all
Images
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All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml
Lane 1 : THP-1 whole cell lysate (ab7913)
Lane 2 : THP-1 whole cell lysate (ab7913) with GFI1 peptide (ab22461) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible cleavage fragment), 36 kDa (possible cross reactivity)ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab21061 has been referenced in 14 publications.
- Costa A et al. Repurposing the lineage-determining transcription factor Atoh1 without redistributing its genomic binding sites. Front Cell Dev Biol 10:1016367 (2022). PubMed: 36420143
- Cheng M et al. Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma. Mol Ther Nucleic Acids 23:418-430 (2021). PubMed: 33473327
- Gu X et al. GFI-1 overexpression promotes cell proliferation and apoptosis resistance in mycosis fungoides by repressing Bax and P21. Oncol Lett 22:521 (2021). PubMed: 34025788
- Huang Y et al. GFI1 promotes the proliferation and migration of esophageal squamous cell carcinoma cells through the inhibition of SOCS1 expression. Int J Mol Med 48:N/A (2021). PubMed: 34368875
- Kucinski I et al. Interactions between lineage-associated transcription factors govern haematopoietic progenitor states. EMBO J 39:e104983 (2020). PubMed: 33103827