Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12263-33] to GFM1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-GFM1 antibody [EPR12263-33]
See all GFM1 primary antibodies
DescriptionRabbit monoclonal [EPR12263-33] to GFM1
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human GFM1. The exact sequence is proprietary.
Database link: Q96RP9
- HeLa, NCI-H460, U87-MG and 293T cell lysates. HeLa cells. Immunoprecipitation pellet from HeLa whole cell lysate (ab150035).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 83 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionMitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
PathwayProtein biosynthesis; polypeptide chain elongation.
Involvement in diseaseDefects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
- Information by UniProt
- COXPD1 antibody
- EF-Gmt antibody
- EFG antibody
All lanes : Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : NCI-H460 cell lysates
Lane 3 : U87-MG cell lysates
Lane 4 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa
Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate: ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.
ab173529 has been referenced in 3 publications.
- Bravo-Alonso I et al. Genes and Variants Underlying Human Congenital Lactic Acidosis-From Genetics to Personalized Treatment. J Clin Med 8:N/A (2019). PubMed: 31683770
- Yokokawa T et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). PubMed: 30369085
- Brito S et al. Long-term survival in a child with severe encephalopathy, multiple respiratory chain deficiency and GFM1 mutations. Front Genet 6:102 (2015). WB . PubMed: 25852744