Recombinant Anti-GFM1 antibody [EPR12263] (ab171945)
![Western blot - Anti-GFM1 antibody [EPR12263] (ab171945)](https://www.abcam.com/ps/products/171/ab171945/Images/ab171945-194530-anti-gfm1-antibody-epr12263-western-blot.jpg "Western blot - Anti-GFM1 antibody [EPR12263] (ab171945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12263] to GFM1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-GFM1 antibody [EPR12263]
See all GFM1 primary antibodies -
Description
Rabbit monoclonal [EPR12263] to GFM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment within Human GFM1. The exact sequence is proprietary.
Database link: Q96RP9 -
Positive control
- HeLa, NCIH460, U87 MG and 293T cell lysates; Human cervical carcinoma tissue and Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12263 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 83 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. -
Pathway
Protein biosynthesis; polypeptide chain elongation. -
Involvement in disease
Defects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 85476 Human
- Omim: 606639 Human
- SwissProt: Q96RP9 Human
- Unigene: 518355 Human
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Alternative names
- COXPD1 antibody
- EF-Gmt antibody
- EFG antibody
see all
Images
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Western blot - Anti-GFM1 antibody [EPR12263] (ab171945)All lanes : Anti-GFM1 antibody [EPR12263] (ab171945) at 1/10000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : NCI 460 cell line lysate
Lane 3 : U87 MG cell line lysate
Lane 4 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] (ab171945)](https://www.abcam.com/ps/products/171/ab171945/Images/ab171945-194533-anti-gfm1-antibody-epr12263-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] (ab171945)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling GFM1 with ab171945 at 1/50
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] (ab171945)](https://www.abcam.com/ps/products/171/ab171945/Images/ab171945-194531-anti-gfm1-antibody-epr12263-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] (ab171945)Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling GFM1 with ab171945 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-GFM1 antibody [EPR12263] (ab171945)](https://www.abcam.com/ps/products/171/ab171945/Images/ab171945-4-benefits-of-recombinant-antibodies.png)
Anti-GFM1 antibody [EPR12263] (ab171945)
Protocols
Datasheets and documents
References (1)
ab171945 has been referenced in 1 publication.
- Simon MT et al. Activation of a cryptic splice site in the mitochondrial elongation factor GFM1 causes combined OXPHOS deficiency. Mitochondrion 34:84-90 (2017). PubMed: 28216230
