Product nameAnti-GFM2 antibody
See all GFM2 primary antibodies
DescriptionRabbit polyclonal to GFM2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Rhesus monkey
Recombinant fragment within Human GFM2 (internal sequence). The exact sequence is proprietary.
Database link: Q969S9
- WB: HEK-293T and HepG2 whole cell extract. ICC/IF: HeLa cells. IHC-P: Mouse testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 87 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceGFM2 is a mitochondrial translation elongation factor. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA.
- EF-G2mt antibody
- EFG2 antibody
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Paraffin-embedded mouse testis tissue stained for GFM2 using ab229463 at 1/1000 dilution in immunohistochemical analysis.
All lanes : Anti-GFM2 antibody (ab229463) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
7.5% SDS-PAGE gel.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GFM2 (green) using ab229463 at 1/500 dilution in ICC/IF. Cells were fixed with 2% paraformaldehyde/culture medium at 37oC for 30 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
ab229463 has not yet been referenced specifically in any publications.