Overview

  • Product name

  • Description

    Rabbit polyclonal to GFM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GFM2 (internal sequence). The exact sequence is proprietary.
    Database link: Q969S9

  • Positive control

    • WB: HEK-293T and HepG2 whole cell extract. ICC/IF: HeLa cells. IHC-P: Mouse testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    GFM2 is a mitochondrial translation elongation factor. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • EF-G2mt antibody
    • EFG2 antibody
    • EFG2mt antibody
    • elongation factor G 2, mitochondrial antibody
    • Elongation factor G2 antibody
    • G elongation factor mitochondrial 2 antibody
    • GFM2 antibody
    • hEFG2 antibody
    • mEFG 2 antibody
    • mEFG2 antibody
    • Mitochondrial elongation factor G2 antibody
    • mitochondrial ribosome recycling factor 2 antibody
    • MRRF2 antibody
    • MST027 antibody
    • MSTP027 antibody
    • OTTHUMP00000222951 antibody
    • OTTHUMP00000222952 antibody
    • ribosome-releasing factor 2, mitochondrial antibody
    • RRF2 antibody
    • RRF2mt antibody
    see all

Images

  • Paraffin-embedded mouse testis tissue stained for GFM2 using ab229463 at 1/1000 dilution in immunohistochemical analysis.

  • All lanes : Anti-GFM2 antibody (ab229463) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 87 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GFM2 (green) using ab229463 at 1/500 dilution in ICC/IF. Cells were fixed with 2% paraformaldehyde/culture medium at 37oC for 30 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229463 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229463.
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