Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Annexin1-GFP)
Total protein in input
25 µg
Specification
HEK 293 cells transfected with Annexin1-GFP
Immuno-precipitation step
Protein G
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% TX-100, 10% glycerol, 1X PBS
Western Blot antibody
Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Additional data
Additional Notes
Line 1: Lysate of HEK 293 cells expressing Annexin1-GFP fusion
Line 2: IP with anti GFP Ab
Line 3: Not bound fraction
Line 2: IP with anti GFP Ab
Line 3: Not bound fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
Submitted Nov 16 2009