Application
Western blot
Sample
Mouse Recombinant protein (HEK293T cells over expressing mouse 5-HT6R)
Loading amount
50 µg
Specification
HEK293T cells over expressing mouse 5-HT6R
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-T (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Pico
Exposure
2 second(s)
Bands
Specific: 75 kDa
Positive control
HEK293T cells overexpressing mouse 5-HT6R
Abcam user community
Verified customer
Submitted Mar 21 2013