Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Drosophila embryo)
Permeabilization
No
Specification
Drosophila embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBT
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa-568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
The abcam anti-GFP antibody was perfect for my experiments. I was able to detect a GFP tagged protein that is is expressed in very small amounts within the large Drosophila embryo with little background or non-specific staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jun 17 2016