Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
25 µg
Specification
Chinese hamster ovary cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Positive control
CHO-K1 cells expressing GFP
Additional data
Additional Notes
Good antibody, non-specific bands are observed though.
Chansik Yoon
Verified customer
Submitted Feb 01 2021