Anti-GFP antibody (ab5449)
Key features and details
- Goat polyclonal to GFP
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-GFP antibody
See all GFP primary antibodies -
Description
Goat polyclonal to GFP -
Host species
Goat -
Specificity
Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Pure GFP protein, or cells known to overexpress GFP.
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General notes
Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. This antibody is available in an affinity purified form as ab5450.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/5000 - 1/20000.
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IP |
Use 0.5µl for 106 cells.
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Notes |
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WB
1/5000 - 1/20000. |
IP
Use 0.5µl for 106 cells. |
Target
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Relevance
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
Images
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Lane 1 : parental YTS cells (negative control)
Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD)KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).
Lane 1 : parental YTS cells (negative control) Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD) KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084). -
5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of:
Lane 1 : 1/2500
Lane 2 : 1/5000
Lane 3 : 1/10,000
Lane 4 : 1/20,000
5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of: Lane 1 : 1/2500 Lane 2 : 1/5000 Lane 3 : 1/10,000 Lane 4 : 1/20,000
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab5449 has been referenced in 14 publications.
- Cui X et al. Mechanism of TREM2/DAP12 complex affecting ß-amyloid plaque deposition in Alzheimer's disease modeled mice through mediating inflammatory response. Brain Res Bull 166:21-28 (2021). PubMed: 33053435
- Kwak MS et al. Reactive oxygen species induce Cys106-mediated anti-parallel HMGB1 dimerization that protects against DNA damage. Redox Biol 40:101858 (2021). PubMed: 33461096
- Rosàs-Canyelles E et al. Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos. Nat Protoc 16:1062-1088 (2021). PubMed: 33452502
- Cerino M et al. Novel CAPN3 variant associated with an autosomal dominant calpainopathy. Neuropathol Appl Neurobiol 46:564-578 (2020). PubMed: 32342993
- El-Bazzal L et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908