Question (18646) | Anti-GFP antibody (ab5449)

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Question

I am trying to detect, by western blotting, a chimeric protein expressed in transgenic plants. The chimeric protein consists of green fluorescent protein (GFP) fused to the carboxy terminus of my protein. I am thinking about buying an anti-GFP antibody but I need to be sure that it will not cross-react with cyan fluorescent protein (CFP).

Answer

Thank you for your enquiry. If your CFP is derived from the GFP of Aequorea victoria jellyfish, then I am afraid all our anti-GFP antibodies will cross-react with that protein, since the difference is a single amino-acid substitution and none of our anti-GFP antibodies are directed against that epitope. If your CFP is from another species, for example the reef coral Anemonia majano, then the antibodies will not cross-react. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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