Western blot abreview for Anti-GFP antibody

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Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (Hansenula polymorpha)
Loading amount
10 µg
Specification
Hansenula polymorpha
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/10000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Goat/Sheep IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
AP substrate kit
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 30 kDa

Additional data

Additional Notes
yGFP expressed in H. polymorpha (yeast) was specifically detected with ab5449 antibody.

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Submitted Sep 28 2009

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