Application
Western blot
Sample
Mouse Cell lysate - whole cell (NMuMG)
Loading amount
50 µg
Specification
NMuMG
Treatment
Hipoxia and also transfected
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBS T
Secondary antibody
Name
Non-Abcam antibody was used: DAKO goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 27 kDa
Additional data
PubMed ID
19955572: View
Additional Notes
In this figure, pGFP C1 vector has been used as transfection control to normalize Sna1 and Fbxl14 expresión.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Raul Peña
Verified customer
Submitted Jan 11 2013