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Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain, Dentate Gyrus Nestin-GFP)
Brain, Dentate Gyrus Nestin-GFP
Antigen retrieval step
Yes - 0.3% Triton X-100
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2 % horse Serum and 0.1 % TX100 in TBS
Non-Abcam antibody was used: Goat-Anti Rabbit IgG
Host species: Goat
Conjugation: Alexa Fluor® 488
Transgenic mice expressing GFP tagged Nestin was used in experiment.
Since the endogenous fluorescence of GFP is less. Anti-GFP antibody was used.
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Submitted Feb 28 2013