Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody

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Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain, Dentate Gyrus Nestin-GFP)
Specification
Brain, Dentate Gyrus Nestin-GFP
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2 % horse Serum and 0.1 % TX100 in TBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat-Anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

PubMed ID
nil: View
Additional Notes
Transgenic mice expressing GFP tagged Nestin was used in experiment.
Since the endogenous fluorescence of GFP is less. Anti-GFP antibody was used.

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Submitted Feb 28 2013

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