Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain, striatal neuron, medium spiny neuron)
Specification
Brain, striatal neuron, medium spiny neuron
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Tween-20
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent

Secondary antibody

Name
Non-Abcam antibody was used: AlexaFlour 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Our lab has been using this antibody for years - it is extremely robust and reliable. The stain is clear, and well-defined. In well-fixed tissue, there is very little background.

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Submitted Mar 05 2013

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