Western blot abreview for Anti-GFP antibody

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Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Dog Cell lysate - whole cell (MDCK cell line)
Specification
MDCK cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS-T Milk 1%
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 29 kDa
Exposure
15 second(s)
Negative control
pcDNA3 transfection
Positive control
peGFP transfection

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Submitted Dec 11 2013

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