IHC - Wholemount abreview for Anti-GFP antibody

Good
Abreviews
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Adult Posterior Midgut, HEAT-FIXED)
Specification
Adult Posterior Midgut, HEAT-FIXED

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Life Technologies A11008
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Fixation by heat as in Miller et al. (1989) J Cell Biol.109:2963-75 but without methanol. Equivalent results with PFA 4% and dilution 1:2000.

Dr. Joaquin Denavascues

Verified customer

Submitted Feb 12 2014

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