Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG, HRP-conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Bands
Specific: 25 kDa
Exposure
10 second(s)
Negative control
Showed no bands.
Positive control
Showed expected bands between 20 and 30 kDa.

Additional data

Additional Notes
Experiment was run on fresh-frozen tissue. Used PVDF membrane. Lysis buffer: 1% SDS with protease inhibitors.

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Submitted Aug 18 2014

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