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Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Whole brain tissue lysate
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent
Non-Abcam antibody was used: anti-rabbit IgG, HRP-conjugate
Host species: Goat
Conjugation: Horse Radish Peroxidase
Showed no bands.
Showed expected bands between 20 and 30 kDa.
Experiment was run on fresh-frozen tissue.
Used PVDF membrane.
Lysis buffer: 1% SDS with protease inhibitors.
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Submitted Aug 18 2014