Western blot abreview for Anti-VEGF antibody

Excellent
Abreviews
Application
Western blot
Sample
Sheep Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: Blocking agent
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
West Pico
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 43 kDa
Positive control
Hela cells

Miss. Maryline Gomes

Verified customer

Submitted Feb 13 2015

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