Western blot abreview for Anti-GFP antibody

Good
Abreviews
Application
Western blot
Loading amount
400000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Specification
Mouse Neuroblastoma (Neuro2A)
Treatment
10% FBS and fibroblast growth factor
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in 1X TBS with 0.1% Tween-20
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-rabbit IgG Antibody from Millipore
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL from WestDura (Thermo Fischer)
Bands
Specific: 26 kDa
Exposure
40 second(s)

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Submitted Apr 01 2015

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