Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung carcinoma cells transfected with CMV-GFP, xen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
Lung carcinoma cells transfected with CMV-GFP, xen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS
Dilution
1/2000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-Rb IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

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Submitted May 17 2016

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