Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Wholemount Embryo)
Permeabilization
Yes - 1% DMSO + 0.5% Triton X
Specification
Wholemount Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+1% BSA+1% DMSO+0.5% TritonX
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Transgenic zebrafish line expressing GFP under a pronephric duct and spinal canal promoter.

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Submitted Jan 03 2017

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