Western blot abreview for Anti-GFP antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
HEK 293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa

Additional data

Additional Notes
Cells lysed in ChIP lysis buffer, crosslinked with 1% formaldehyde

Abcam user community

Verified customer

Submitted Apr 23 2018

Sign up