Western blot abreview for Anti-GFP antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
15 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS/0.01% Tween-20
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: HRP AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
HeLa cell lysate (lane 1)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 28 kDa Non-specific: 34 kDa
Positive control
Lysate from HeLa cells stably expressing 3 different GFP-tagged protein constructs (lanes 2, 3 & 4) or GFP only (lane 5).

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Submitted Feb 06 2019

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