Anti-GFP antibody (ab6673)
- Datasheet
- References (203)
- Protocols
Overview
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Product nameAnti-GFP antibody
See all GFP primary antibodies -
DescriptionGoat polyclonal to GFP
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Host speciesGoat
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SpecificityAnti-GFP assayed by ELISA for direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP.
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Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, IHC-P, IHC-FrFl, IHC-Frmore details
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Immunogen
Fusion protein corresponding to Aequorea victoria GFP aa 1-246.
Database link: P42212 -
Positive control
- HeLa nuclear extract lysate (ab150036) can be used as a positive control in WB. E5.5 Hex-GFP transgenic mouse embryo.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab6673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/400 - 1/2000. (for immunoprecipitated GFP, see Abreview). |
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| IP | Use at an assay dependent concentration. | |
| ELISA | 1/40000. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. |
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| ICC/IF | 1/500. | |
| IHC-P | 1/200 - 1/1000. | |
| IHC-FrFl | Use at an assay dependent concentration. | |
| IHC-Fr | 1/200 - 1/1000. |
Target
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RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)This image is courtesy of Bart RountreeImmunofluorescence of TGN mouse liver labeling GFP on hepatocytes with ab6673.
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Western blot - Anti-GFP antibody (ab6673)All lanes : Anti-GFP antibody (ab6673) at 1 µg/ml
Lane 1 : HeLa cells
Lane 2 : mock transfected HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-GFP antibody (ab6673)This image is courtesy of an anonymous abreview.All lanes : Anti-GFP antibody (ab6673) at 1/1000 dilution
Lane 1 : MRC5VA lung fibroblast whole cell lysate overexpressing EGFP alone
Lanes 2-3 : MRC5VA lung fibroblast whole cell lysate overexpressing an EGFP fusion protein
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated anti-goat polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 27,55 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab6673)This image is courtesy of Jeff KleinImmunohistochemistry of GFP transgenic mouse liver labeling GFP with ab6673.
References
This product has been referenced in:
- Goldman SM et al. Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury. PLoS One 13:e0191245 (2018). Read more (PubMed: 29329332) »
- Campbell K et al. Differential roles of the Drosophila EMT-inducing transcription factors Snail and Serpent in driving primary tumour growth. PLoS Genet 14:e1007167 (2018). Read more (PubMed: 29420531) »
