Western blot abreview for Anti-GFP antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS/Tween

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Goat-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: <35kDa kDa
Positive control
MRC5VA Cells overexpressing EGFP alone and 2 EGFP-tagged proteins

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Submitted Feb 21 2011

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